Table 1

Primers used for real-time-quantitative polymerase chain reaction in porcine pulmonary tissue

GenePrimer sequences
HPRT
 Sense5′-tcaggcagtataatccaaagatggt-3′
 Antisense5'-agtctggcttatatccaacacttcg-3'
ET-1
 Sense5'-tcctgctcttccctgatgga-3'
 Antisense5'-tgctcaggagtgttggaccca-3'
5-HT1B
 Sense5'-gacccgagcccaactgataa-3'
 Antisense5'-gtgaccgaagatgtggaccc-3'
5-HT1D
 Sense5'-atagtaaacgccggacagcg-3'
 Antisense5'-agacaatggcaatcatggca-3'
5-HT2B
 Sense5'-tgtgccatttcagtggatcg-3'
 Antisense5'-ggcctggattggctttttg-3'
5-HT4
 Sense5'-ccgtgctgatctcctttctcc-3'
 Antisense5'-tgccgatgttattccagcct-3'
5-HTT
 Sense5'-tggaacactggcaactgcac-3'
 Antisense5'-ccaggtgatgttgtcctcgg-3'
  • HPRT: hypoxanthine-guanine phosphoribosyl transferase

  • ET‐1: endothelin 1

  • 5‐HT1B, 5‐HT1D, 5‐HT2B, 5‐HT4: serotonin receptors

  • 5‐HTT: serotonin transporter