Characterisation of surface molecules on exosome-coated beads
| BALF exosomes# | MDDC exosomes# | |||
| MFI+ | Experiments¶ n | MFI+ | Experiments¶ n | |
| HLA‐DR | 20.6 (17.0–33.4) | 7 | 63.6 (49.3–117.3) | 6 |
| MHC class I | 12.7 (9.8–14.3) | 3 | 18.2 (10.2–34.8) | 3 |
| CD3 | 10.1 (10.0–10.2) | 3 | 10.1 (9.9–10.1) | 4 |
| CD14 | 9.5 (9.4–9.9) | 3 | 9.9 (9.7–10.3) | 5 |
| CD40 | 10.2 (10.1–10.2) | 3 | 12.8 (11.9–16.9) | 5 |
| CD54 | 12.0 (11.5–12.3) | 3 | 16.0 (13.6–16.2) | 3 |
| CD63 | 31.6 (26.8–38.0) | 3 | 17.5 (13.4–39.6) | 6 |
| CD80 | 9.9, 10.1 | 2 | 10.8 (10.5–12.4) | 5 |
| CD86 | 12.1 (11.7–13.0) | 3 | 18.7 (15.1–21.3) | 5 |
All data presented as median (range) unless otherwise stated
Values >10.2 were considered positive
BALF: bronchoalveolar lavage fluid
MFI: mean fluorescence intensity
MDDC: monocyte-derived dendritic cell
HLA‐DR: human leukocyte antigen‐DR
MHC: major histocompatibility complexes
#: exosomes from BALF taken from healthy individuals and exosomes from monocyte-derived dendritic cells cultured for 7 days from healthy blood donors were coated to anti‐MHC class II magnetic beads and analysed by flow cytometric analysis for the expression of surface molecules
A minimum of 5×103 beads per sample were examined
¶: number of independent experiments using exosomes from different donors
+: the MFI for isotype-matched controls were normalised to 10 fluorescence units 15