EM concentration µg·mL−1 | ||||
0 | 0.05 | 0.5 | 5 | |
Viable count ×109 cfu·mL−1 | 4.9±6.54 | 10.0±15.84 | 9.7±15.69 | 4.12±4.25 |
Log viable count | 9.5±0.44 | 9.5±0.76 | 9.5±0.78 | 9.3±0.627 |
Bacilli in 20 SEM fields# | 542.6±426.20* | 202.2±163.34* | 220.9±150.99* | 200.1±127.77* |
Adherence density | 56.8±43.16* | 21.5±17.56* | 23.3±14.65* | 21.4±12.65* |
Data are shown as mean±sd
Cfu: colony-forming units
#: total number
Adherence density was calculated as the ratio of the total number of bacilli in 20 random scanning electron microscopy (SEM) fields to log viable count for each of the individual experiments
*: p<0.05 when compared with data obtained with no EM
n=12