Antibodies, lectins and kits used to investigate parenchymal alterations in rat lungs instilled with recombinant human keratinocyte growth factor
| Marker | Main specificity | Type of marker | Origin | Dilution, antigen retrieval | Detection system |
| CC10 | Clara cells | Rabbit antirat polyclonal antibody | Dr. Klug (Marburg, Germany) | 1:1000 Microwave | Peroxidasecoupled goat antirabbit IgG |
| CGRP | Neuroendocrine cells | Mouse antirat monoclonal antibody (clone C4/19) | Sigma/RBI | 1:100 Microwave | ABC |
| SP‐D | Clara cells, alveolar type II cells, alveolar macrophages | Mouse antirat monoclonal antibody (clone S7) | S. Albrecht (Dresden, Germany) | Undiluted Microwave | Peroxidasecoupled goat antirabbit IgG |
| Pancytokeratin | Pulmonary epithelial cells | Mouse antihuman monoclonal antibody (clone MNF116) | Dako | 1:150 Pronase | APAAP |
| Cytokeratin 18 | Airway brush cells, alveolar type III cells | Mouse antihuman monoclonal antibody (clone Ks18.04) | Progen | 1:20 Pronase | ABC |
| CD44v6 | Airway epithelial cells, alveolar type II cells | Mouse antirat monoclonal antibody (clone 1.1 AMSL) | U. Günthert (Basel, Switzerland) | Undiluted | ABC |
| BrdU | Proliferating cells (S‐phase) | Mouse antirat monoclonal antibody (clone Bu20a) | Dako | 1:100 Microwave | ABC |
| Ki67 | Proliferating cells (cell cycle) | Mouse antirat monoclonal antibody (MIB‐5) | Dianova | 1:5 Microwave | ABC |
| TUNEL | Apoptotic cells | TdT, dUTP | Oncor, Appligene | DAB |
CC10: Clara cell specific protein 10
CGRP: calcitonin generelated peptide
SP‐D: surfactant protein D
BrdU: 5‐bromo‐2′‐deoxyuridine
TUNEL: terminal deoxynucleotidyl transferasemediated deoxyuridine triphosphate nick endlabelling
TdT: terminal deoxynucleotidyl transferase
dUTP: deoxyuridine triphosphate
Ig: immunoglobulin
ABC: avidinbiotin peroxidase complex
APAAP: alkaline phosphataseantialkaline phosphatase labelling
DAB: diaminobenzidine