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Antibodies, lectins and kits used to investigate parenchymal alterations in rat lungs instilled with recombinant human keratinocyte growth factor

MarkerMain specificityType of markerOriginDilution, antigen retrievalDetection system
CC10Clara cellsRabbit anti­rat polyclonal antibodyDr. Klug (Marburg, Germany)1:1000 MicrowavePeroxidase­coupled goat anti­rabbit IgG
CGRPNeuroendocrine cellsMouse anti­rat monoclonal antibody (clone C4/19)Sigma/RBI1:100 MicrowaveABC
SP‐DClara cells, alveolar type II cells, alveolar macrophagesMouse anti­rat monoclonal antibody (clone S7)S. Albrecht (Dresden, Germany)Undiluted MicrowavePeroxidase­coupled goat anti­rabbit IgG
Pan­cytokeratinPulmonary epithelial cellsMouse anti­human monoclonal antibody (clone MNF116)Dako1:150 PronaseAPAAP
Cytokeratin 18Airway brush cells, alveolar type III cellsMouse anti­human monoclonal antibody (clone Ks18.04)Progen1:20 PronaseABC
CD44v6Airway epithelial cells, alveolar type II cellsMouse anti­rat monoclonal antibody (clone 1.1 AMSL)U. Günthert (Basel, Switzerland)UndilutedABC
BrdUProliferating cells (S‐phase)Mouse anti­rat monoclonal antibody (clone Bu20a)Dako1:100 MicrowaveABC
Ki­67Proliferating cells (cell cycle)Mouse anti­rat monoclonal antibody (MIB‐5)Dianova1:5 MicrowaveABC
TUNELApoptotic cellsTdT, dUTPOncor, AppligeneDAB
  • CC10: Clara cell specific protein 10

  • CGRP: calcitonin gene­related peptide

  • SP‐D: surfactant protein D

  • BrdU: 5‐bromo‐2′‐deoxyuridine

  • TUNEL: terminal deoxynucleotidyl transferase­mediated deoxyuridine triphosphate nick end­labelling

  • TdT: terminal deoxynucleotidyl transferase

  • dUTP: deoxyuridine triphosphate

  • Ig: immunoglobulin

  • ABC: avidin­biotin peroxidase complex

  • APAAP: alkaline phosphatase­antialkaline phosphatase labelling

  • DAB: diaminobenzidine