Transcriptionally regulated genes in tumour necrosis factor (TNF)‐α stimulated BEAS‐2B cells revealed by cDNA array analysis after normalisation of the two arrays against housekeeping genes represented on the array membrane
| TNF‐α upregulation | x‐fold |
| IL‐6 | 9.8# |
| IL‐8 | 16.5# |
| MCP‐1 | 18.0# |
| GRO-α | 10.1# |
| GRO-β | 9.2# |
| GRO-γ | 6.1# |
| RANTES | 10.9# |
| ICAM‐1 | 11.1# |
| GM‐CSF | 6.1# |
| IL‐7R | 4.2# |
| IL‐1β | 5.4# |
| CD70 | 5.7# |
| Integrin 5α | 3.9 |
| M‐CSF | 2.3 |
| HCC1 | 2.3 |
| IP10 | 2.8 |
| Eotaxin | 2.5 |
Values represent x‐fold regulation versus control as measured by OptiQuantTM phosphoimaging software by Packard Instruments Company
IL: interleukin
MCP: monocyte chemoattractant protein
GRO: growth-related oncogene
ICAM: intercellular adhesion molecule
GM‐CSF: granulocyte-monocyte colony-stimulating factor
M‐CSF: monocyte colony-stimulating factor
HCC: human CC cytokine
IP: interferon-gamma inducible protein
RANTES: regulated on activation, normal T‐cell expressed and secreted
#: regulation was further characterised and verified by real-time polymerase chain reaction