Source and working dilution of the antibodies
| Antibodies | Source | Working dilution | Chromogenic substrate |
| Rabbit antihuman CTGF | Okayama University | 1:500 | DAB |
| Mouse antihuman CD68 (PGM1) | DAKO Gostrup, Denmark | 1:100 | DAB |
| Mouse antihuman cytokeratin | Nichirei Co, Japan | 1:200 | DAB |
| Mouse antihuman α-SMA | Nichirei Co, Japan | 1:200 | DAB |
CTGF: connective tissue growth factor
α-SMA: α-smooth muscle actin
DAB: 3′3-diaminobenzidine