Univariable models | Multivariable model | |||||||
Estimated coefficient | CI 2.5% | CI 97.5% | p-value | Estimated coefficient | CI 2.5% | CI 97.5% | p-value | |
Dependent variable: asthma control (ACQ6 score) | ||||||||
Sex | −0.036 | −0.35 | 0.28 | 0.82 | 0.033 | −0.28 | 0.35 | 0.84 |
Age at donation | 0.016 | 0.005 | 0.027 | 0.005 | ||||
BMI kg·m–2 | 0.040 | 0.015 | 0.066 | 0.002 | 0.041 | 0.015 | 0.067 | 0.004 |
CLEC4C mRNA | −0.071 | −1.3 | 1.1 | 0.91 | ||||
TLR7 mRNA | −0.12 | −1.3 | 1.0 | 0.83 | 0.88 | −0.42 | 2.2 | 0.18 |
TLR8 mRNA | −1.0 | −2.1 | 0.12 | 0.079 | −1.4 | −2.7 | −0.093 | 0.036 |
IFNα ng·mL−1 | −0.024 | −0.17 | 0.12 | 0.74 | ||||
TNF ng·mL−1 | −0.021 | −0.060 | 0.017 | 0.28 | ||||
IL12 ng·mL−1 | −0.063 | −0.51 | 0.39 | 0.78 | ||||
Dependent variable: asthma severity (GINA step) | ||||||||
Sex | 1.0 | 0.53 | 1.9 | 0.98 | 1.4 | 0.70 | 2.7 | 0.36 |
Age at donation | 1.04 | 1.02 | 1.07 | <0.001 | 1.03 | 1.01 | 1.06 | 0.005 |
BMI kg·m–2 | 1.1 | 1.1 | 1.2 | <0.001 | 1.1 | 1.0 | 1.2 | 0.004 |
CLEC4C mRNA | 0.13 | 0.010 | 1.2 | 0.093 | ||||
TLR7 mRNA | 0.13 | 0.015 | 1.0 | 0.057 | 0.23 | 0.024 | 2.0 | 0.19 |
TLR8 mRNA | 0.19 | 0.021 | 1.5 | 0.12 | ||||
IFNα ng·mL−1 | 0.85 | 0.62 | 1.1 | 0.39 | ||||
TNF ng·mL−1 | 0.96 | 0.88 | 1.0 | 0.73 | ||||
IL12 ng·mL−1 | 1.5 | 0.70 | 4.1 | 0.36 |
ACQ: Asthma Control Questionnaire; GINA: Global Initiative for Asthma. Multivariable model included n=150. Multivariable model included n=147 with 3 missing observations deleted. Adjusted R2: 0.06, p=0.010. CLEC4C, Toll-like receptor (TLR)7 and TLR8 gene expression at baseline were measured in resting whole blood, interferon (IFN)α production stimulated with RV16, tumour necrosis factor (TNF) and interleukin (IL)12 production stimulated with TLR8 agonist were measured in peripheral blood mononuclear cells.