TABLE 2

Differentially expressed proteins between severe asthma current smokers (CSA) and healthy nonsmokers (NH) and severe asthma nonsmokers (NSA) and NH by sputum assay

Probe IDProtein targetGene symbolGene nameFunction
CSA-NH    
 SL001726CSF2CSF2 (or GM-CSF)Colony-stimulating factor 2Granulocyte, monocyte, macrophage expansion
 SL004925AGR2AGR2Anterior gradient protein 2Mucin (MUC5AC and MUC5B) overproduction in asthma
Localised in endoplasmic reticulum of bronchial epithelial cells
 SL000039IL-8CXCL8 (=IL8)C-X-C motif chemokine ligand 8Acts as one of the major mediators of the inflammatory response by recruiting neutrophils
NSA-NH    
 SL000342CatalaseCATCatalaseA key antioxidant enzyme in the body's defence against oxidative stress
 SL000051CRPCRPC-reactive proteinHost defence based on its ability to recognise foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood
 SL004153M-CSF RCSF1RColony-stimulating factor 1 receptorThe receptor for CSF-1, a cytokine which controls the production, differentiation and function of macrophages
 SL004853B7-H2ICOSLGInducible T-cell costimulatory ligandThis protein acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces B-cell proliferation and differentiation into plasma cells
 SL006108CD5LCD5LCD5 molecule likeThis secreted protein is mainly expressed by macrophages in lymphoid and inflamed tissues and regulates mechanisms in inflammatory responses. Regulation of intracellular lipids mediated by this protein has a direct effect on transcription regulation mediated by nuclear receptors ROR-γ (RORC)
 SL004068GZMBGZMBGranzyme BTargets cell lysis in cell-mediated immune responses. Processes cytokines and degrades extracellular matrix proteins; these roles are implicated in chronic inflammation and wound healing
CSA-NH and NSA-NH
 SL017613FCG2A/BFCGR2AFc fragment of immunoglobulin-γ receptor IIaThe protein is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes
 SL003524Protein disulfide isomerase A3PDIA3Protein disulfide isomerase family A member 3Formation of the final antigen conformation, export from the endoplasmic reticulum to the cell surface and adaptation to oxidative damage

Assay performed by SomaLogic (Boulder, CO, USA). GM-CSF: granulocyte-macrophage colony-stimulating factor; IL: interleukin.