Assessment of in vivo pulmonary artery pressure and in vitro reactivity
| Vehicle | Sildenafil 1 mg·kg−1 | Two-way ANOVA main effects p-value | |||||
| Sham-exposed | CS-exposed | Sham-exposed | CS-exposed | CS exposure | Sildenafil | Interaction | |
| Guinea-pigs n | 8 | 10 | 8 | 10 | |||
| Pulmonary artery pressure mmHg | |||||||
| Systolic | 9.0±4.3 | 14.3±8.0* | 6.6±2.0 | 8.2±2.7# | 0.082 | 0.038 | 0.346 |
| Diastolic | 3.8±2.5 | 10.5±7.5 | 5.0±1.2 | 4.9±1.6 | 0.059 | 0.202 | 0.050 |
| Mean | 5.7±1.6 | 12.4±7.7* | 5.7±1.5 | 6.3±1.8# | 0.036 | 0.081 | 0.081 |
| Contraction mg | |||||||
| KCl 60 mM | 1828.4±489.2 | 2560.9±711.1* | 1660.7±530.1 | 2604.9±605.7* | ≤0.001 | 0.771 | 0.619 |
| Noradrenaline 10−6 M | 574.6±163.1 | 573.5±200.2 | 538.1±105.7 | 717.0±172.0 | 0.141 | 0.370 | 0.136 |
| Relaxation in response to ADP | |||||||
| Change in tension AUC | 203.8±20.1 | 231.8±43.3 | 200.6±57.4 | 226.3±27.2 | 0.058 | 0.753 | 0.935 |
| EC50 -log [M] ADP | 7.18±0.86 | 6.61±0.40 | 6.79±0.60 | 6.72±0.27 | 0.096 | 0.479 | 0.191 |
Data are presented as mean±sd, unless otherwise stated. CS: cigarette smoke; KCl: potassium chloride; ADP: adenosine diphosphate; AUC: area under the curve; EC50: median effective concentration. *: p<0.05 compared with sham-exposed group; #: p<0.05 compared with vehicle CS-exposed group.