Table 1– Pre-analytical procedures involved in clinical studies of circulating microparticles (MPs) in obstructive sleep apnoea

First author [ref.]	Year	Anticoagulant	Preparation of PPP	Sedimentation of MPs	Storage	Wash step
Geiser [49]	2002	Citrate	No	No	Formaldehyde	No
Jelic [50]	2009	Citrate	160×g, 10 min; 1000×g, 6 min	No	−80°C	No
Ayers [51]	2009	Citrate	1550×g, 20 min	18 000×g, 30min	−80°C	Yes
Priou [52]	2010	EDTA	270×g, 20 min; 1500×g, 20 min	No	−80°C	No
Yun [53]	2010	Citrate	1500×g, 15 min; 13 000×g, 2 min	No	−70°C	No
Kim [54]	2011		2000×g, 20 min; 20 000×g, 90 min	No	−80°C	No
Maruyama [52]	2012	EDTA-ACD	8000×g, 5 min	No	−20°C	No

PPP: platelet-poor plasma; ACD: acid–citrate–dextrose.