Primer | Gene information | Sequence |
RT-PCR | ||
Bak | NM_001188 | F: 5′-GACGACATCAACCGACGCTATG-3′ R: 5′-GCTGGTGGCAATCTTGGTGAAG-3′ |
Bcl-xL | NM_138578 | F: 5′-ACTGTGCGTGGAAAGCGTAGAC-3′ R: 5′GATCCAAGGCTCTAGGTGGTCATTC-3′ |
DNMT1 | NM_001379 | F: 5′-CCGTCTCTTGAAGGTGGTGTTAATG-3′ R: 5′-ATGAGGTGCTGAAGCCGATGAG-3′ |
DNMT3a | NM_175630 | F: 5′-GCACCACGGCACGGAAGG-3′ R: 5′-GGACTTGGAGATCACCGCAGG-3′ |
18S | NR_003278 | F: 5′-GTCTGCCCTATCAACTTTCG-3′ R:5′-ATGTGGTAGCCGTTTCTCA-3′ |
ChIP and methylation | ||
Bak | ENSG00000030110 | F: 5′-ACTGGAGTCTCGCGGGTCCC-3′ R: 5′-CAACCCGGGTGGCTCAGCAG-3′ |
Bcl-xL | ENSG00000171552 | F: 5′-GGCTTGTTCGGGAGAGACGGC-3′ R: 5′-CCCCCTTCATCGGCCCGGTAG-3′ |
DNMT1: DNA methyltransferase 1: DNMT3a: DNA methyltransferase 3a; ChIP: chromatin immunoprecipitation.