Table 1– Serum lysyl oxidase-like 2 (LOXL2) immunoassay performance characteristics
Assay characteristicResult
Accuracy relative error<15%
Intra-assay precision coefficient of variation5%
Inter-assay precision coefficient of variation25%
LLOD180 pg·mL−1
LLOQ440 pg·mL−1
Upper limit of quantitationAbsolute limit not determined; highest calibrator standard used was 132 118 pg·mL−1
  • Accuracy was determined using pooled serum (from healthy individuals) spiked with recombinant LOXL2 protein. Intra-assay and inter-assay precision were determined using incurred samples. Lower limit of detection (LLOD) is the mean +2.5×sd of the blank wells. Lower limit of quantitation (LLOQ) is the lowest calibrator standard with relative error <30% and coefficient of variation <30%.