| Primers | Sequence | Nucleotide position | Target | GATC-site position | Amplification conditions | |
| Inverse PCR | ||||||
| TTMV-LY-9F | 5'-GACCACAAACCGTCACTTAGTTCC-3' | 42–65 | Betatorquevirus | NA | 95°C for 1 min 40 cycles: 95°C for 30 s 54°C for 1 min 72°C for 3 min 72°C for 10 min | |
| TTMV-LY-8R | 5'-GCAATTTAAATTAGGTGGTTTTCCTG-3' | 40–15 | ||||
| TTMV-LY-11F | 5'-CTAGRTCAGTCTGGCGGAACGG-3' | 288–309 | Betatorquevirus | NA | ||
| TTMV-LY-10R | 5'-CCCCTTGACTACGGTGGTTTCAC-3' | 278–256 | ||||
| Real-time PCR | ||||||
| TTMV-LY-F | 5'-ATTCGAATGGCTGAGTTTATGC-3' | 170–191 | Betatorquevirus | 210, 579, 2674 | 95°C for 3 min 35 cycles: 95°C for 10 s 54°C for 30 s 72°C for 34 s | |
| TTMV-LY-R | 5'-CCTTGACTACGGTGGTTTCAC-3' | 276–256 | ||||
| TTMV-LY-P | 5'-CCAGACGGAGACCGGATCACTTCA-3' (FAM-BHQ1) | 194–217 | ||||
| TTMV-LY-2F | 5'-CACGAATTAGCCAAGACTGGGCAC-3' | 2398–2421# | TTMV-LY2 | 512, 1382, 2456, 2732, 2746# | ||
| TTMV-LY-2R | 5'-TGCAGGCATTCGAGGGCTTGTT-3' | 2494–2473# | ||||
| TTMV-LY-2P | 5'-ACAGAGCCAGATCGACAGCAACTGCT-3' (FAM-BHQ1) | 2445–2470# | ||||
Nucleotide position and GATC-sites are indicated based on TGP96 (AB041962) sequence. TTMV-LY: torque teno mini virus isolates; NA: not applicable; F: forward; R: reverse; P: probe. #: nucleotide positions are based on TTMV-LY2 sequence.