Table 2 Pulmonary cytokine and chemokine levels in wild-type (WT) mice and mice lacking the lectin-like domain of thrombomodulin (TM^LeD/LeD) at 6, 24 and 48 h post-infection with pneumococcal pneumonia

	6 h post-infection		24 h post-infection		48 h post-infection
	WT	TM^LeD/LeD	WT	TM^LeD/LeD	WT	TM^LeD/LeD
KC ng·mL⁻¹	ND	ND	1.19 (0.72–3.10)	1.10 (0.96–1.42)	11.4 (10.2–15.1)	3.70 (1.79–9.79)*
MIP-2 ng·mL⁻¹	ND	ND	3.66 (3.16–4.52)	3.38 (2.99–3.81)	11.8 (6.65–16.6)	2.77 (2.23–5.54)**
TNF-α pg·mL⁻¹	20.2 (12.7–26.0)	23.1 (15.5–30.1)	5.17 (2.50–6.31)	2.50 (2.50–13.1)	116 (83.9–150)	8.10 (4.24–39.7)*
IL-6 pg·mL⁻¹	36.4 (25.4–41.5)	44.8 (30.5–67.2)	19.1 (5.40–123)	7.63 (5.38–18.3)	889 (501–1174)	58.8 (17.0–594)***
IL-10 pg·mL⁻¹	6.03 (5.13–6.69)	6.74 (6.26–7.72)	2.50 (2.50–5.36)	3.75 (2.50–5.38)	8.20 (2.50–17.9)	2.50 (2.50–2.50)*
IL-12 pg·mL⁻¹	BD	BD	BD	BD	11.3 (5.00–16.1)	5.00 (5.00–10.1)
IFN-γ pg·mL⁻¹	1.46 (1.40–1.57)	1.68 (1.36–2.22)	2.19 (2.03–3.59)	2.58 (1.96–3.19)	23.6 (14.2–35.1)	3.78 (2.46–16.7)*
MCP-1 ng·mL⁻¹	299 (222–341)	315 (278–620)	0.19 (0.15–0.24)	0.19 (0.18–0.30)	7.18 (4.51–10.1)	0.95 (0.64–6.10)*

Data are presented as median (interquartile range). Pneumonia was induced by intranasal inoculation with 5×10⁴ Streptococcus pneumoniae CFU. KC: keratinocyte-derived chemokine; MIP-2: macrophage inflammatory protein-2; TNF-α: tumour necrosis factor-α; IL: interleukin; IFN-γ: interferon-γ; MCP-1: monocyte chemotactic protein-1; ND: not determined; BD: below detection. n=8 per group in all cases per time-point. *: p<0.05; **: p<0.01; ***: p<0.001, statistical significance compared with WT using Mann–Whitney U-test.