RT Journal Article
SR Electronic
T1 Intermittent hypoxia alters gut microbiota diversity in a mouse model of sleep apnoea
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1055
OP 1065
DO 10.1183/09031936.00184314
VO 45
IS 4
A1 Isabel Moreno-Indias
A1 Marta Torres
A1 Josep M. Montserrat
A1 Lidia Sanchez-Alcoholado
A1 Fernando Cardona
A1 Francisco J. Tinahones
A1 David Gozal
A1 Valeryi A. Poroyko
A1 Daniel Navajas
A1 Maria I. Queipo-Ortuño
A1 Ramon Farré
YR 2015
UL http://erj.ersjournals.com/content/45/4/1055.abstract
AB We assessed whether intermittent hypoxia, which emulates one of the hallmarks of obstructive sleep apnoea (OSA), leads to altered faecal microbiome in a murine model.In vivo partial pressure of oxygen was measured in colonic faeces during intermittent hypoxia in four anesthetised mice. 10 mice were subjected to a pattern of chronic intermittent hypoxia (20 s at 5% O2 and 40 s at room air for 6 h·day−1) for 6 weeks and 10 mice served as normoxic controls. Faecal samples were obtained and microbiome composition was determined by 16S rRNA pyrosequencing and bioinformatic analysis by Quantitative Insights into Microbial Ecology.Intermittent hypoxia exposures translated into hypoxia/re-oxygenation patterns in the faeces proximal to the bowel epithelium (<200 μm). A significant effect of intermittent hypoxia on global microbial community structure was found. Intermittent hypoxia increased the α-diversity (Shannon index, p<0.05) and induced a change in the gut microbiota (ANOSIM analysis of β-diversity, p<0.05). Specifically, intermittent hypoxia-exposed mice showed a higher abundance of Firmicutes and a smaller abundance of Bacteroidetes and Proteobacteria phyla than controls.Faecal microbiota composition and diversity are altered as a result of intermittent hypoxia realistically mimicking OSA, suggesting the possibility that physiological interplays between host and gut microbiota could be deregulated in OSA.Faecal microbiota composition and diversity are altered due to intermittent hypoxia mimicking OSA in a murine model http://ow.ly/ERjA9