@article {LeickP964, author = {Edna A. Leick and Bruno Tadeu Martins Oliveira and Leandro V. Oliva and Rafael Almeida-Reis and Osmar A. Theodoro and M.L.V. Oliva and I.F.L.C. Tib{\'e}rio}, title = {Plant proteinase from bauhinia bauhinioides kallikrein inhibitor (BbKI) attenuates inflammation and remodelling induced by elastase in mice}, volume = {44}, number = {Suppl 58}, elocation-id = {P964}, year = {2014}, publisher = {European Respiratory Society}, abstract = {Aims: To evaluate if a plant Kunitz proteinase inhibitor BbKI contributes to inactivation of elastase-induced inflammatory and extracellular matrix remodelling alterations. Methods: C57Bl6 mice received elastase (50ml/animal/intratracheal-E-group) or saline (Ve-group). Mice were treated with BbKI (2mg/kg) on days 1, 14, 21 after elastase instillation (I-E group) or saline instillation (I-Ve group). On day 30 mice were anesthetized, mechanically ventilated, lungs were removed and we quantified neutrophils, positive cells for MMP-9, MMP-12, TNF-a, MAC-2, TIMP, IL8, MUC5, iNOS, eNOS and volume fraction of elastic and collagen fibers and PGF2alpha in alveolar septa and airways. Results: In E-group, there was an increase of neutrophils, MMP-9, MMP-12, TNF-a, MAC-2, TIMP, IL8, MUC5, iNOS, eNOS, elastic and collagen fibers and isoprostanes in alveolar and/or airways compared to controls (p\<0.05). In I-E group, there was a reduction in alveolar septa of neutrophils, positive cells for MMP-9, MMP-12, TNF-a, MAC-2, TIMP1, IL8, iNOS, eNOS, PGF2alpha, collagen and elastic fibers compared to E-group. Considering airways of I-E group, there was a decrease of neutrophils, positive cells for MMP-9, MMP-12, TNF-a, TIMP1, IL8, MUC5, iNOS, eNOS, PGF2alpha, elastic and collagen fibers compared to E-group (p\<0.05). Conclusions: This proteinase inhibitor (BbKI) reduced elastase-induced pulmonary inflammatory and extracellular matrix remodeling alterations induced by elastase and this inhibitor may contribute as potential therapeutic tool for COPD management. Financial Support: FAPESP, CNPq, LIM-20 HC-FMUSP.}, issn = {0903-1936}, URL = {https://erj.ersjournals.com/content/44/Suppl_58/P964}, eprint = {https://erj.ersjournals.com/content}, journal = {European Respiratory Journal} }