RT Journal Article
SR Electronic
T1 Comparison of <em>aspergillus</em> precipitin with quantitative <em>aspergillus</em> IgG assay
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 4644
VO 44
IS Suppl 58
A1 Satoru Fujiuchi
A1 Yuka Fujita
A1 Hokuto Suzuki
A1 Tomoaki Aritomi
A1 Hikaru Kuroda
A1 Masaaki Takahashi
A1 Akinori Takeda
A1 Yasuhiro Yamazaki
A1 Tadakatsu Tsuji
A1 Toshiaki Fujikane
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/4644.abstract
AB Background: Detection of IgG antibodies to Aspergillus fumigatus is important for diagnosing chronic pulmonary aspergillosis (CPA). Immunodiffusion assay has been widely used for detecting circulating anti-Aspergillus antibodies (precipitin), however, it appears to lack sensitivity. Recently, quantitative measurement of IgG to Aspergillus by using fluorescent immunoenzyme assay (ImmunoCapTM) is commercially available. In this study, we examined performance of ImmunoCapTM with respect to diagnosis of CPA.Method: We detected serum Aspergillus precipitin in patients with following condition; 1) complaining subacute to chronic respiratory and/or systemic symptom, 2) developing radiological shadow in lung (cavitation, infiltration and thickness of pleura) within 3-6 months, and 3) existence of underlying chronic respiratory disease. These patients were divided to four groups (proven, probable, possible and control) according to classification criteria. We also examined specific IgG to Aspergillus for these collected sera by ImmunoCapTM.Result: From January 2007 to August 2013, 353 patients were studied. Aspergillus IgG values was significantly higher in the proven and probable groups than in the possible and control groups (p &amp;lt;0.01). ROC analysis revealed that cut off value of ImmunoCapTM for diagnosis of proven and probable cases was 50 mgA/L (AUC, 0.83; 95% CI, 0.786-0.874; sensitivity, 0.79; specificity, 0.74 ).Conclusions: The sensitivity and specificity of ImmunoCapTM for diagnosis of CPA were reliable. Thus, ImmunoCapTM may alternate with conventional precipitin test for diagnosing CPA. In addition, the quantitation of IgG might be useful for monitoring marker of antifungal treatment response.