PT  - JOURNAL ARTICLE
AU  - Hadice Selimoglu Sen
AU  - Velat Sen
AU  - Mehtap Bozkurt
AU  - Gül Türkçü
AU  - Ibrahim Kaplan
AU  - Özlem Abakay
AU  - Cengizhan Sezgi
AU  - Abdulmenap Güzel
TI  - Carvacrol and pomegranate extract in treating methotrexate-induced lung oxidative injury in rats
DP  - 2014 Sep 01
TA  - European Respiratory Journal
PG  - P3917
VI  - 44
IP  - Suppl 58
4099  - http://erj.ersjournals.com/content/44/Suppl_58/P3917.short
4100  - http://erj.ersjournals.com/content/44/Suppl_58/P3917.full
SO  - Eur Respir J2014 Sep 01; 44
AB  - Introduction and Objectives: Methotrexate (MTX), a folic acid analogue, is commonly used over a wide range of doses for various therapeutic applications. This study was designed to evaluate the effects of carvacrol (CRV) and pomegranate extract (PE) on MTX induced lung injury in rats.Materials and Methods: A total of 32 male rats were subdivided into 4 groups: control (group I), MTX treated (group II), MTX+CRV treated (group III), and MTX+PE treated (group IV). A single dose of 73 mg/kg CRV was administered intraperitoneally to rats in group III on Day 1 of the investigation. To group IV, a dose of 225 mg/kg of PE was administered via orogastric gavage once daily over seven days. A single dose of 20 mg/kg of MTX was given intraperitoneally to groups II, III and IV on Day 2. The total duration of experiment was 8 days. Malondialdehyde (MDA), total oxidant status (TOS), total antioxidant capacity (TAC), and oxidative stress index (OSI) were measured from rat lung tissues and cardiac blood samples.Results: Serum and lung specimen analyses demonstrated that MDA, TOS and OSI levels were significantly greater in group II relative to controls. Conversely, the TAC level was significantly reduced in group II when compared to the control group. Pre-administering either CRV or PE was associated with decreased MDA, TOS, and OSI levels and increased TAC levels compared to rats treated with MTX alone. Histopathological examination revealed that lung injury was less severe in group 3 and 4 relative to group 2.Conclusions: MTX treatment results in rat lung oxidative damage that is partially counteracted by pretreatment with either CRV or PE.