RT Journal Article
SR Electronic
T1 Bronchial provocation testing and collection of sputum with inhaled mannitol in children
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P3524
VO 44
IS Suppl 58
A1 Gerardo Vizmanos-Lamotte
A1 Elisa Canturri Gispert
A1 Victor Núñez Eroles
A1 Mireia Martínez Abelló
A1 Joan Martínez Benazet
A1 Manuel Medina Rams
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/P3524.abstract
AB IntroductionMannitol is used as an indirect challenge test to assess airway hyperresponsiveness in asthma. Recently, it has been tested in adults as an sputum inducer (Wood, L.G. et al. Clin Exp Allergy 2010; 40:232-41).The aim of this preliminary study was to assess the feasibility and safety of the mannitol challenge test to obtain induced sputum in young children.Methods17 children (10 healthy and 7 asthmatics) aged 7-13 years were recruited. The mannitol challenge test (Osmohale®; Aldo-Unión, Esplugues de Ll., Barcelona, Spain) was performed as previously published and induction sputum was done during the test. Sputum was processed using a slight modification of the method described by Pizzichini et al. (Pizzichini, E. et al. Eur Respir J 1996; 9:1174-80). Sputum eosinophlia was established when the percentage of eosinophils was ≥ 2.5%.ResultsMannitol test was positive in 7 children (4 asthmatic and 3 healthy), provoking dose to induce a 15% fall in FEV1 (PD15) ranged from 75 to 565.38 mg and the response-dose ratio (RDR: % fall in FEV1/cumulative dose) ranged from 0.03 to 0.14% fall in FEV1/mg. Sputum samples (viability > 50%; squamous cells < 20%; weight ranged from 0.1 to 0.38g) were obtained in 15 children. Eosinophilia was present in 7 samples (5 asthmatic children). Adverse events were mild.ConclusionsMannitol can be used to induce good-quality sputum in young children.