TY - JOUR T1 - Polymorphism of <em>HLA-G</em> gene promotor in patients with non small cell lung cancer JF - European Respiratory Journal JO - Eur Respir J VL - 44 IS - Suppl 58 SP - P2706 AU - Aneta Kowal AU - Andrzej Wisniewski AU - Piotr Kusnierczyk AU - Renata Jankowska Y1 - 2014/09/01 UR - http://erj.ersjournals.com/content/44/Suppl_58/P2706.abstract N2 - Introduction: HLA-G is a nonclassical MHC class I molecule which is expressed only in immunoprivileged tissues. HLA-G expression on cancer cells makes them resistant to cytotoxicity of NK cells and cytotoxic lymphocytes T. The HLA-G gene polymorphism is very limited.Aims: The aim of the study was to establish the role of HLA-G gene polymorphism in patients with non small cell lung cancer. We analised two single nucleotide polimorphisms (SNP) in the promotor region of HLA-G gene: -725C&gt;G&gt;T and -716T&gt;G.Methods: 143 patients with diagnosis of non small cell lung cancer were enroled to the study. Blood samples were collected before any oncological therapies. The TGGE method (temperature gradient gel electrophoresis) was used to obtain different band patterns of the HLA-G gene promoter fragment. We observed the answer to the treatment and the course of the disease.Results: All genotyped SNPs were in Hardy-Weinberg equilibrum. We didn't prove a relationship between the genotype -725C&gt;G&gt;T or -716T&gt;G and the risk of lung cancer compared to heatlhy volunteers. There was no connection between the two SNPs and the survival time or the histological type of cancer. Although there was no impact of the examined genotypes on the T feature, we found the association of genotype -725C&gt;G&gt;T and the range of lymph nodes metastases (N feature). We noticed also a tendency to worse answer to therapy patients with more C allels in -725 position and patients with more T allels in -716 position.Conclusions: In our study we couldn't prove the relationship between the examined SNPs and the course of the disease because of too small patient samples but this need to be examined as different trends were shown. ER -