PT  - JOURNAL ARTICLE
AU  - Meenakshi Ponnana
AU  - Ramya Sivangala
AU  - Lavanya Joshi
AU  - Shruthi Thada
AU  - Vijaya Lakshmi Valluri
AU  - Suman latha Gaddam
TI  - Effect of vitamin D levels on natural killer cells in diabetes mellitus patients with tuberculosis
DP  - 2014 Sep 01
TA  - European Respiratory Journal
PG  - P2685
VI  - 44
IP  - Suppl 58
4099  - http://erj.ersjournals.com/content/44/Suppl_58/P2685.short
4100  - http://erj.ersjournals.com/content/44/Suppl_58/P2685.full
SO  - Eur Respir J2014 Sep 01; 44
AB  - It is increasingly apparent that Natural Killer(NK) cells play crucial role in innate defense mechanism against Mycobacterium tuberculosis(M.tb).Diabetes mellitus (DM) is a predisposing factor that alters immunity to Tuberculosis(TB).Further NK cell function depends on adequate levels of Vitamin D, an important regulator of immune system.A total of 150 subjects,TB patients(TB),DM patients with TB(TBDM),their household contacts (HHC),DM patients and Healthy Controls(HC) 25 each in which,CD4,CD8,CD16 & CD56 cell counts were enumerated.Vitamin D levels in serum,IFN-γ & IL-10 levels in M.tb antigen (Ag 85A)stimulated culture supernatants were estimated by ELISA(pg/ml) and ARMS-PCR was carried out to study the SNP's using specific primers. CD56 & CD16 NK cells were significantly low in TBDM patients,their HHC & and DM patients with deficient levels of vitamin D compared to HC (p<0.005) indicating that HHC are prone to develop the disease.The mean IFN-γ cytokine levels were significant for (TB 6.74±4.1423.66±14.96; p<0.0004 & TBDM 6.17±4.42; 32.08±8.8 p<0.03); for IL-10 (9.88±3.45; 34.18±11.74 p<0.04 in TB 70.74±16.5; 14.37±4.14 p<0.01 in TBDM & 69.01±15.29; 13.99±3.9; p<0.01 in DM) compared to HC. AA & AT genotypes of IFN-γ (p<0.009; 95% C.I 0.266-0.853; P<0.032 95% C.I 1.038-2.653 in TB & P< 0.037 95% C.I. 0.261-0.991; p<0.001 95% C.I. 1.349-4.208 in DM); GA & GG genotypes of IL-10 -1082G/A were significantly associated in all the subjects (p< 0.0001). In HHC of the patients, low serum Vitamin D levels and decrease cell count may predispose to TB infection. Hence the study may help in identifying the high risk individuals.