RT Journal Article
SR Electronic
T1 The composition of diesel exhaust particles affects differently the cell signaling and cytoskeleton in bronchial epithelial cells
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P3897
VO 44
IS Suppl 58
A1 Macchione, Mariangela
A1 Seriani, Robson
A1 Junqueira, Mara S.
A1 Toledo, Alessandra C.
A1 Martinez, Diana
A1 Alencar, Adriano M.
A1 Martins, Milton A.
A1 Saldiva, Paulo H.N.
A1 Negri, Elnara M.
A1 Mauad, Thais
YR 2014
UL http://erj.ersjournals.com/content/44/Suppl_58/P3897.abstract
AB Backgroung: Diesel exhaust particles (DEP) have a major contribution of the ultrafine particles to the air pollution. Hydrocarbons and metals adsorbed on surface of DEP cause physiological and molecular disturbances in cells of the respiratory system. Methods: We used four samples of DEP with different compositions after treatment: 1) Acid extraction (HNO365%) to remove mainly metals (DEP/NA); 2) solvents: a) methanol to remove organic composed with high and medium polarity (DEP/MET), b) hexane to remove organic compound with low polarity (DEP/MET) and DEP crude to study MAPKs activation (ERK and JNK) and cell rheology (OMTC technical). The cells were exposed to 100µg/mL of DEPs fractions in air-liquid system during 15, 30 and 60 minutes. The MAPKs JNK and ERK are activated simultaneously in DEP crude and DEP/MET. Results: In DEP/NA and DEP/HEX only JNK was activated. The compositions of DEP/MET are mainly iron, cupper and cadmium and Benz[a]anthracene and pyrene. DEP/NA and DEP/HEX had a reduction of 50% of PHAs concentration, but the copper and cadmium appear to be the most available causing JNK activation, significant (p=0.03) reduction in fiber coherence and increased viscoelasticity at short time (15 minutes). Conclusion: The adverse findings detected after exposure to DEPs fractions represents the toxic potential of diesel compounds and could be consequence of using bad fuel with low-technology engines. In addition the activation of these MAPKs is a major indication that the chemical composition of the DEP around the world can influence the onset of or worsening of respiratory diseases via MAPKs activation.