PT  - JOURNAL ARTICLE
AU  - Patrick Geraghty
AU  - Jules Dabo
AU  - Itsaso Garcia-Arcos
AU  - Neville Cummins
AU  - Robert Foronjy
TI  - Late-breaking abstract: Late-breaking abstract: E-cigarette exposure induces pathological responses that result in lung tissue destruction and airway hyper reactivity in mice
DP  - 2014 Sep 01
TA  - European Respiratory Journal
PG  - 3435
VI  - 44
IP  - Suppl 58
4099  - http://erj.ersjournals.com/content/44/Suppl_58/3435.short
4100  - http://erj.ersjournals.com/content/44/Suppl_58/3435.full
SO  - Eur Respir J2014 Sep 01; 44
AB  - E-cigarettes emit less particulates and toxins than standard cigarettes but they still expose users to a multitude of potentially harmful compounds. Given their growing use, there is a strong public health need to assess the safety and lung effects of e-cigarettes. This study developed an in vivo model of e-cigarette exposure in A/J mice using a small animal nebulizer. Cohorts of mice (n=8 per group) were exposed for 1 hour/day, 5 days a week for 4 months to either nebulized PBS, vehicle (50% propylene glycol/50% vegetable glycerin), e-cigarette liquid (American eLiquid Store) containing 18 mg/ml nicotine in 50%PG/50% VG or e-cigarette liquid containing 36 mg/ml nicotine in 50% PG/50% VG. Exposure to e-cigarette vapor with nicotine increased lung cytokine and protease expression, mucin staining in the airways, caspase 3/7 activity in the tissue and TUNEL staining in the lung parenchyma.In addition, e-cigarettes induced emphysema and airway hyperreactivity while the vehicle had no effect.Thus, this study shows for the first time that e-cigarette exposure causes asthma and emphysema in vivo.