TY - JOUR T1 - Preventive and therapeutic treatment with a7 nicotinic receptor agonist reduced lung inflammation and collagen deposition in mice with acute lung injury JF - European Respiratory Journal JO - Eur Respir J VL - 44 IS - Suppl 58 SP - P3926 AU - Nathalia Pinheiro AU - Fernanda Santana AU - Marina Guerreiro AU - Luciana Caperuto AU - Iolanda Tiberio AU - Vânia Prado AU - Marco Antonio Prado AU - Milton Martins AU - Carla Prado Y1 - 2014/09/01 UR - http://erj.ersjournals.com/content/44/Suppl_58/P3926.abstract N2 - Although cholinergic activity is involved in airway tone control, particularly by stimulation of muscarinic receptors, there is evidence that cholinergic anti-inflammatory pathway modulates immune systemic inflammatory responses in different models.Aim: We evaluated whether a7-nicotinic acetylcholine receptor (a7nAChR)stimulation reverted lung inflammation observed in an experimental model of acute lung injury (ALI).Methods:Male C57/BL6 mice received PNU-282987 (specificα7nAChRagonist) 30 min before (10 mg/kg, ip) or 6 hours after (2.5; 5 or 10 mg/kg) LPS administration (5 mg/kg, intratraqueal). We evaluated pulmonary mechanics, bronchoalveolar lavage fluid (BALF), collagen fibers and activation of NF-kBby immunohistochemistry and by Western Blotafter 24 hours from LPS.Results:After six hours, LPS treated animals presented increase in neutrophils and macrophages in BALF. Both pre and pos-treatment with PNU (10mg/Kg) reduced the total cells, macrophagesand neutrophilsin BALF (p<0.001 for all comparisons) as well as the NF-kB protein expression and positive cells (p<0.05) compared to LPS animals treated with vehicle. In addition, the PNU pretreatment attenuated the volume proportion of collagen fibers in lung tissue induced by LPS instillation (p<0.01) Conclusion: We concluded that a7 nAChRstimulation prevents and revertslung inflammation in a model of ALI probably by controlling NF-kB activation. Our data reinforced the idea that a7 nAChRis an important pathway to be better explored in the treatment of acute respiratory distress syndrome. ER -