RT Journal Article SR Electronic T1 Effects of cigarette smoke extract on human airway smooth muscle cells in COPD JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP 634 OP 646 DO 10.1183/09031936.00171313 VO 44 IS 3 A1 Ling Chen A1 Qi Ge A1 Gavin Tjin A1 Hatem Alkhouri A1 Linghong Deng A1 Corry-Anke Brandsma A1 Ian Adcock A1 Wim Timens A1 Dirkje Postma A1 Janette K. Burgess A1 Judith L. Black A1 Brian G.G. Oliver YR 2014 UL http://erj.ersjournals.com/content/44/3/634.abstract AB We hypothesised that the response to cigarette smoke in airway smooth muscle (ASM) cells from smokers with chronic obstructive pulmonary disease (COPD) would be intrinsically different from smokers without COPD, producing greater pro-inflammatory mediators and factors relating to airway remodelling. ASM cells were obtained from smokers with or without COPD, and then stimulated with cigarette smoke extract (CSE) or transforming growth factor-β1. The production of chemokines and matrix metalloproteinases (MMPs) were measured by ELISA, and the deposition of collagens by extracellular matrix ELISA. The effects of CSE on cell attachment and wound healing were measured by toluidine blue attachment and cell tracker green wound healing assays. CSE increased the release of CXCL8 and CXCL1 from human ASM cells, and cells from smokers with COPD produced more CSE-induced CXCL1. The production of MMP-1, -3 and -10, and the deposition of collagen VIII alpha 1 (COL8A1) were increased by CSE, especially in the COPD group which had higher production of MMP-1 and deposition of COL8A1. CSE decreased ASM cell attachment and wound healing in the COPD group only. ASM cells from smokers with COPD were more sensitive to CSE stimulation, which may explain, in part, why some smokers develop COPD. Cigarette smoke extract induces airway remodelling-associated changes in airway smooth muscle cells in COPD patients http://ow.ly/wK0ey