RT Journal Article
SR Electronic
T1 Etiologies of acute exacerbation of bronchial asthma in adults by real-time polymerase chain reaction
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P848
VO 42
IS Suppl 57
A1 Kenichiro Shimizu
A1 Yutaka Yoshii
A1 Miyuki Morozumi
A1 Naoko Chiba
A1 Kimiko Ubukata
A1 Hiromichi Hara
A1 Takanori Numata
A1 Makoto Kawaishi
A1 Keisuke Saito
A1 Jun Araya
A1 Katsutoshi Nakayama
A1 Kazuyoshi Kuwano
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/P848.abstract
AB Background: Most common microorganisms associated with acute exacerbation of bronchial asthma (AEBA) are respiratory viruses such as rhinovirus (RV) and atypical bacteria such as Mycoplasma pneumoniae (M. p). Causative organisms of AEBA in pediatric populations have been well documented; however, these have been rarely reported in adults. Recently multiplex polymerase chain reaction (PCR) has been applied to detect effectively both respiratory bacteria and viruses. Aims: To evaluate etiologies in adult AEBA, a rapid reliable process based on real-time PCR for respiratory samples was used. Methods: From September 2012 to January 2013, we prospectively enrolled adult AEBA patients who satisfied our criteria; 20 years of age or older, within 7 days of onset, and informed consent. Nasopharyngeal swab and sputum samples were collected from all patients and comprehensive real-time PCR was used to detect 6 bacteria and 11 respiratory viruses. Results: Among 36 patients who satisfied our criteria, 25 (69.4%) of them detected microorganisms both bacteria and/or viruses by PCR test. 7 (19.4%) were diagnosed with viral infection, 11 (30.6%) with bacterial infection, 3 (8.3%) with atypical bacterial infection, and 4 (11.1%) with viral/bacterial co-infection. The remaining 11 (30.6 %) had unknown pathogens. Most leading microorganisms were Haemophilus influenzae (H. i), M. p, and RV. Conclusions: Our results suggest usefulness of real-time PCR for nasopharyngeal swab and sputum sample in estimating etiologies in adult AEBA. Results of the detection of M. p and RV were as expected; however, H. i was unpredicted. Based on the results, we analyzed the association between microorganisms and AEBA.