PT  - JOURNAL ARTICLE
AU  - Harpreet Lota
AU  - Carmel Stock
AU  - Hiroe Sato
AU  - Carmen Fonseca
AU  - Athol Wells
AU  - Christopher Denton
AU  - David Abraham
AU  - Gisela Lindahl
AU  - Elisabetta Renzoni
TI  - LSC 2013 abstract - Hypoxia-inducible factor 1 alpha polymorphisms in relation to pulmonary involvement in systemic sclerosis
DP  - 2013 Sep 01
TA  - European Respiratory Journal
PG  - 3522
VI  - 42
IP  - Suppl 57
4099  - http://erj.ersjournals.com/content/42/Suppl_57/3522.short
4100  - http://erj.ersjournals.com/content/42/Suppl_57/3522.full
SO  - Eur Respir J2013 Sep 01; 42
AB  - Introduction: Transcription factor hypoxia-inducible factor 1 (HIF1) upregulates the expression of angiogenic genes including vascular endothelial growth factor. Systemic sclerosis (SSc) is a multisystem disease characterised by organ fibrosis and widespread vasculopathy. We assessed the association of two transcriptionally active, single nucleotide polymorphisms (SNPs) of the hypoxia-inducible factor 1 alpha gene (HIF1A) in SSc.Methods: Using a case-control study design, HIF1A SNPs (rs11549465 C/T, rs12434438 A/G) were genotyped in SSc patients (n=426) and UK controls from the Wellcome Trust Case-Control Consortium (n=5564 and 5659). The SSc cohort was subdivided according to the presence or absence of pulmonary fibrosis (PF) (n=227 v. 199), pulmonary hypertension (PH) on echocardiography (n=17 v. 129) and autoantibody subsets; anti-topoisomerase I antibodies (n=108) and anti-centromere antibodies (n=105).Results: Genotype distribution in all groups conformed to Hardy-Weinberg equilibrium. There were no significant differences in genotype or allele frequencies between SSc and controls (SSc: CC:82.1%, CT:16.7%, TT:1.2% v. controls: CC:81.3%, CT:17.8%, TT:0.9% and SSc: AA:63.9%, AG:32.6%, GG:3.5% v. controls: AA:63.4%, AG:32.5%, GG:4.1%). On subgroup analysis, no significant differences were observed according to PF, autoantibodies, or in the group with available echocardiography (n=146), according to PH.Conclusion: An association was not detected between HIF1A SNPs and SSc. Due to limited power in the PH subgroup, an association with pulmonary vascular involvement cannot be excluded and requires further evaluation and longitudinal analysis.