TY - JOUR T1 - Aquoaporin-1 expression in idiopathic pulmonary fibrosis JF - European Respiratory Journal JO - Eur Respir J VL - 42 IS - Suppl 57 SP - P2337 AU - César Gutiérrez AU - Álvaro Donate AU - Lourdes Gómez Izquierdo AU - María Molina-Molina AU - Miriam Echevarría AU - Jose Antonio Rodriguez Portal Y1 - 2013/09/01 UR - http://erj.ersjournals.com/content/42/Suppl_57/P2337.abstract N2 - INTRODUCTION: Idiopathic pulmonary fibrosis (IPF) is an interstitial lung disease. IPF is the result of alveolar reepitalization and interstitial fibroblasts proliferation. Aquaporin-1 is a membrane protein that helps to the water transport between alveolar space and lung parenquima. AQP1 could contribute in cellular proliferative process in IPF. Our objective was to determine AQP1 expression in different lung cellular types in patients with IPF.METHODS: we studied by immunohistochemistry with AQP1 antibodies various histological sections corresponding to diverse lung diseases. They were included normal lung, hypersensitivity pneumonitis, sarcoidosis and IPF samples. The stain analysis was cualitative, and it was made by two independent observers. We gave 0: no stain, + (25% of the sample), ++ (50% of the sample), +++ (75% of the sample) and ++++ (100% of the sample). It was analyzed which cells showed AQP1 on their surface.RESULTS:IPF: type II pneumocytes hyperplasia, which show AQP1 on their surface, a 75% of visual field (+++). Type I pneumocytes did not show AQP1 on their surface. Plenty of collagen fibers and fibroblasts without AQP1 expression.Hypersensitivity Pneumonitis: type II pneumocytes with AQP1 expression on their surface. AQP1 with a 5% by field (0/+).Sarcoidosis: 0% cuantitative AQP1 expression.Normal lung: almost absolute type I pneumocytes predominance, without AQP1 expression.We noticed AQP1 expression in all erytrocites and endotellium cells, in all analyzed samples.CONCLUSIONS: In IPF, hyperplasic type II pneumocytes show AQP1 on their surface. For this reason, these hyperplastic cells and the regulation of AQP1 could be implicated in the physiopathology of IPF. ER -