PT  - JOURNAL ARTICLE
AU  - Im, Hanna
AU  - Hirota, Jeremy
AU  - Rahman, Md. Mostafizur
AU  - Rumzhum, Nowshin
AU  - Manetsch, Melanie
AU  - Pascoe, Chris
AU  - Oliver, Brian
AU  - Ammit, Alaina
TI  - The NLRP3 inflammasome is not activated in airway smooth muscle upon TLR2 ligation
DP  - 2013 Sep 01
TA  - European Respiratory Journal
PG  - P559
VI  - 42
IP  - Suppl 57
4099  - http://erj.ersjournals.com/content/42/Suppl_57/P559.short
4100  - http://erj.ersjournals.com/content/42/Suppl_57/P559.full
SO  - Eur Respir J2013 Sep 01; 42
AB  - Inflammasomes have emerged as playing key roles in inflammation and innate immunity. A growing body of evidence has suggested that the nucleotide-binding domain and leucine-rich repeat protein 3 (NLRP3) inflammasome is important in chronic airway diseases such as asthma and COPD. Inflammasome activation results, in part, in pro-IL-1β processing and secretion of the pro-inflammatory cytokine IL-1β. Because asthma exacerbations are associated with elevated levels of secreted IL-1β we addressed whether the NLRP3 inflammasome is activated under in vitro conditions that mimic infectious exacerbation in asthma. Primary cultures of airway smooth muscle (ASM) cells were treated with infectious stimuli (mimicked using the TLR2 agonist Pam3CSK4, a synthetic bacterial lipopeptide). While Pam3CSK4 robustly upregulated ASM cytokine expression in response to TNFα and significantly enhanced IL-1β mRNA expression, we were unable to detect IL-1β in the cell supernatants. Thus, IL-1β was not secreted and therefore unable to act in an autocrine manner to promote amplification of ASM inflammatory responses. Moreover, TLR2 ligation did not enhance NLRP3 mRNA expression in ASM cells, nor was NLRP3 protein detected in the airway smooth muscle layer of tracheal sections from human donors. In conclusion, these data demonstrate that enhanced synthetic function of ASM cells, induced by infectious exacerbation of airway inflammation, is NLRP3 inflammasome and IL-1β-independent. Activation of NLRP3 inflammasome by invading pathogens may prove cell-type specific in exacerbation of airway inflammation in asthma.