TY - JOUR T1 - Dialysis plus ultrafiltration significantly improves the detection of cytokines in induced sputum JF - European Respiratory Journal JO - Eur Respir J VL - 42 IS - Suppl 57 SP - P829 AU - Costel Agache AU - Beate Rückert AU - Ioana Agache AU - Cristina Ciobanu AU - Cezmi Akdis Y1 - 2013/09/01 UR - http://erj.ersjournals.com/content/42/Suppl_57/P829.abstract N2 - BackgroundMeasurement of cytokines in induced sputum proves difficult due to sputum composition (mucus, proteases) and processing (DTT addition, filtration, dilution).Research hypothesisDTT eluting followed by sputum concentration will improve cytokine detection.MethodsStudy was approved by the Local Ethics Committee. Sputum induction and processing (ERS recommendations) was identical for two groups of adult asthmatics. Before cytokine detection (Bio-Plex Pro Human Cytokine 27-plex, Bio-Rad) the sputum was processed for group I by concentration with Centricon Ultracel YM-3 membrane (Millipore, 3000 NMWL) and for group II by dialysis (D-Tube Dialyzers Midi, MWCO 3.5 kDa, Novagen) followed by ultrafiltration (UF) - Amicon Ultra 3K, Millipore, 3000 NMWL. Data were analyzed with SPSS 21.ResultsGroup I: 11 patients, mean age 52.73±12.87 years old, 36% females; group II: 24 patients, mean age 47.75±15.57 years old, 79% females.Dialysis + UF lead to a significant better detection ratio (figure 1) and in higher concentrations (figure 2) of sputum cytokines.ConclusionDialysis plus ultrafiltration proves a valuable method for measuring sputum cytokines. ER -