RT Journal Article
SR Electronic
T1 Dialysis plus ultrafiltration significantly improves the detection of cytokines in induced sputum
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P829
VO 42
IS Suppl 57
A1 Costel Agache
A1 Beate Rückert
A1 Ioana Agache
A1 Cristina Ciobanu
A1 Cezmi Akdis
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/P829.abstract
AB BackgroundMeasurement of cytokines in induced sputum proves difficult due to sputum composition (mucus, proteases) and processing (DTT addition, filtration, dilution).Research hypothesisDTT eluting followed by sputum concentration will improve cytokine detection.MethodsStudy was approved by the Local Ethics Committee. Sputum induction and processing (ERS recommendations) was identical for two groups of adult asthmatics. Before cytokine detection (Bio-Plex Pro Human Cytokine 27-plex, Bio-Rad) the sputum was processed for group I by concentration with Centricon Ultracel YM-3 membrane (Millipore, 3000 NMWL) and for group II by dialysis (D-Tube Dialyzers Midi, MWCO 3.5 kDa, Novagen) followed by ultrafiltration (UF) - Amicon Ultra 3K, Millipore, 3000 NMWL. Data were analyzed with SPSS 21.ResultsGroup I: 11 patients, mean age 52.73±12.87 years old, 36% females; group II: 24 patients, mean age 47.75±15.57 years old, 79% females.Dialysis + UF lead to a significant better detection ratio (figure 1) and in higher concentrations (figure 2) of sputum cytokines.ConclusionDialysis plus ultrafiltration proves a valuable method for measuring sputum cytokines.