RT Journal Article SR Electronic T1 Nitrosative and oxidative stress in virus-induced COPD exacerbations JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP P1892 VO 42 IS Suppl 57 A1 Joseph Footitt A1 Patrick Mallia A1 Wanxing Eugene Ho A1 Chang Cheng A1 Hong Yong Peh A1 Maria-Belen Trujillo-Torralbo A1 Ian Adcock A1 Peter Barnes A1 Kazuhiro Ito A1 Sarah Elkin A1 Onn Min Kon A1 Fred Wong A1 Sebastian Johnston YR 2013 UL http://erj.ersjournals.com/content/42/Suppl_57/P1892.abstract AB Background. Nitrosative and oxidative stress are involved in the pathogenesis of COPD but their role in acute exacerbations of COPD (AECOPD) is unclear. We used experimental rhinovirus (RV) infection to examine the role of RNS and oxidative stress in AECOPD.Methods. 9 COPD subjects, 9 smokers (SMK) and 8 non-smokers (NS) were successfully infected with RV. Induced sputum (IS) was collected prior to infection and on days 3, 5, 9, 12, 15, 21 and 42 post-infection. Markers of nitrosative and oxidative stress 3-nitrotyrosine (3-NT), 8-hydroxy-2'-deoxyguanosine (8-OHDG) and 8-isoprostane, and inflammatory mediators were measured in IS supernatants.Results. At baseline 3-NT levels were significantly higher in the COPD group compared to both NS (P<0.001) and SMK (P<0.05). Post-RV infection 3-NT levels increased significantly on day 9 in the COPD and SMK groups. 8-isoprostane increased in the COPD group on days 9 (P<0.001) and 15 (P<0.05) and in the SMK on day 9 (P<0.05). 8OHdG increased in the COPD group on days 9 and 15 (P<0.05) and in the SMK on day 9 (P<0.05). 8OHdG in the COPD group was significantly higher compared to the SMK on day 15 (P<0.05). In the COPD group peak 8OHdG levels correlated with peak sputum neutrophil elastase (NE) (r=0.68, P=0.042). Peak 3-NT levels correlated with NE (r=0.82, P=0.0072), 8OHdG (r=0.88, P=0.0016), IL-1β (r=0.73, P=0.025), MMP-9 (r=0.83, P=0.0053) and TNF-α (r=0.0125, P=0.78).Conclusions. Following RV infection nitrosative and oxidative stress are increased in COPD. The marker of nitrosative stress 3-NT correlates with levels of inflammatory mediators in sputum. Oxidative and nitrosative stress are potential targets for the development of novel therapies for AECOPD.