PT  - JOURNAL ARTICLE
AU  - Diandian Li
AU  - Ting Yang
AU  - Yongchun Shen
AU  - Shujin Guo
AU  - Fuqiang Wen
TI  - Quercetin attenuates inflammation in cigarette smoke stimulated airway epithelial cells: Possible involvement of autophagy
DP  - 2013 Sep 01
TA  - European Respiratory Journal
PG  - P1590
VI  - 42
IP  - Suppl 57
4099  - http://erj.ersjournals.com/content/42/Suppl_57/P1590.short
4100  - http://erj.ersjournals.com/content/42/Suppl_57/P1590.full
SO  - Eur Respir J2013 Sep 01; 42
AB  - Objectives: Cigarette smoke is known to cause inflammatory response in human bronchial epithelial cells. In this study, we investigated the effects of quercetin, a flavonoid abundant in fruits and vegetables, on cigarette smoke extract (CSE)-induced inflammation in human bronchial epithelial cells (HBEC) and roles of autophagy in this process.Methods: HBEC were exposed to CSE (4%) for 24h, with or without pretreatment of quercetin (10μM or 20μM). The autophagy activation marker, LC3 II protein, was detected with western blotting. Autophagy was inhibited by 3-methyladenine (3-MA) (2.5mM). TNF-α and IL-1β levels in the supernatant were determined by ELISA.Results: Exposure of HBEC to CSE significantly increased TNF-α and IL-1β expression and this upregulaion was significantly attenuated by quercetin (p<0.05). Meanwhile, the pre-treatment of quercetin decreased the ratio of LC3 II to LC3 I, which was increased by CSE (p<0.05). Inhibiting autophagy by 3-MA also decreased TNF-α and IL-1β levels to a similar level as that of quercetin.Conclusion: These results suggest that quercetin attenuated inflammation induced by cigarette smoke. The anti- inflammatory effect might act through modulating autophagy.