RT Journal Article
SR Electronic
T1 Serum from patients with sarcoidosis promotes the proliferation of epithelial cell lines in vitro
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P696
VO 40
IS Suppl 56
A1 Zampira, Iris
A1 Gogali, Athena
A1 Oikonomidis, Petros
A1 Ntzani, Evaggelia
A1 Evagelou, Agellos
A1 Tachmatzoglou, Fanny
A1 Matthaiou, Maria
A1 Canavaros, Panagiotis
A1 Constantopoulos, Stavros
A1 Dalavanga, Yotanna
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P696.abstract
AB INTRODUCTION: Several epidemiological studies have linked sarcoidosis with neoplasia, but this is questioned by many investigators. The present study is an experimental approach to the subject. Our hypothesis was that if sarcoidosis can influence one of the two basic steps of malignancy: cancerous transformation of the cells or out of control cell growth, this could be regarded as an indirect sign of relation to neoplasia.MATERIALS AND METHODS: A549 and SKMES cell lines were treated with serum from patients with sarcoidosis, COPD and healthy individuals. Their effect on cell proliferation was examined. The influence of cytokines and EGFR on cell proliferation was also investigated.RESULTS: When serum from patients with sarcoidosis was added to the culture medium of both epithelial cell lines, there was a statistically significant increase of cell proliferation (p<0.05). By contrast, serum from normal controls or patients with COPD had no impact. This positive influence of serum from patients with sarcoidosis was annulated by EGFR inhibitor.CONCLUSION: Out of control cell growth is a basic step towards malignancy. The increase of epithelial cell proliferation in the presence of sarcoidosis serum, documented in the present study, could represent a link of this disease with neoplasia, under favorable circumstances.