@article {HassounP2364, author = {Dorian Hassoun and David Lair and Virginie Forest and Marie-Aude Cheminant and Christine Sagan and Julie Chesn{\'e} and Karine Botturi-Cavaill{\`e}s and Damien Reboulleau and Patricia Lemarchand and Antoine Magnan}, title = {Immune modulation by mesenchymal stem cells in a mouse model of house dust mite allergic asthma}, volume = {40}, number = {Suppl 56}, elocation-id = {P2364}, year = {2012}, publisher = {European Respiratory Society}, abstract = {Background: Mesenchymal Stem Cells (MSCs) display immunomodulatory features in ovalbumin-induced allergic asthma models, by inducing Th1 and/or Treg polarization. However, little is known about their role in pneumallergen adjuvant free animal models.Objectives: Our aim was to evaluate whether MSC could suppress inflammation in an adjuvant-free mouse model of house dust mite (HDM) allergic asthma. We hypothesized that alleviated inflammatory responses could trigger a decrease in airway hyper responsiveness (AHR) in treated mice.Methods: After epicutaneous sensitization to HDM on day 0, 7, 14 and 21, mice were challenged with intranasal HDM on day 27 and 34. Asthmatic and sham non-asthmatic mice received an I.V. injection of 5.105 MSCs immediately prior first challenge. Effects on AHR, pulmonary inflammation, bronchoalveolar lavage (BAL), cytokine secretion and serum immunoglobulin were assessed on day 35 and 37.Results: Treated asthmatic mice displayed a 1,8 fold decrease of airway resistance estimated by PENH (p\<0,05) compared to controls with a return to baseline. Moreover, asthmatic mice treated with MSC had significantly fewer infiltrated cells in BAL than control mice (308{\textpm}34 vs 734{\textpm}119, N.103/mL, p\<0.001), especially neutrophils (p\<0.01), T cells (p\<0,05) and B cells (p\<0.05). An increase of IL-10 in BAL was observed in treated mice compared with controls (26,80{\textpm}11,19 vs 10,39{\textpm}2,75, pg/mL). In parallel, non-asthmatic treated mice showed neither significant pulmonary cell infiltration (227{\textpm}38 vs 152{\textpm}18, N.103/mL) nor development of AHR compared to controls.Conclusions: Taken together, these results demonstrate that MSC reduced AHR in a relevant allergic model of HDM asthma.}, issn = {0903-1936}, URL = {https://erj.ersjournals.com/content/40/Suppl_56/P2364}, eprint = {https://erj.ersjournals.com/content/40/Suppl_56/P2364.full.pdf}, journal = {European Respiratory Journal} }