PT - JOURNAL ARTICLE AU - Natalia Zakharchuk AU - Vera Nevzorova AU - Tatyana Brodskay AU - Elena Gonchar AU - Irina Agafonova TI - Vasomotor activity of the cerebral arteries in rats with experimental COPD DP - 2012 Sep 01 TA - European Respiratory Journal PG - P1065 VI - 40 IP - Suppl 56 4099 - http://erj.ersjournals.com/content/40/Suppl_56/P1065.short 4100 - http://erj.ersjournals.com/content/40/Suppl_56/P1065.full SO - Eur Respir J2012 Sep 01; 40 AB - The aim of the study was to evaluate the endotelium-dependent and endotelium-independent reactions of the cerebral arteries reproduced in vivo COPD model. The COPD model was reproduced by chronic smoking in rats within 6 months according to H.Zheng protocol. The experimental group consisted of six male Wistar rats. The control group breathed only clean air. Assessment of the endothelial vasomotor reactions were carried out in 6 months after the experiment start and in 2 months after smoking cessation. Acetylcholine and nitroglycerin were injected in the rats body to assess endothelium-dependent (EDVD) and endothelium-independent (EIDVD) vasodilation, N-monomethyl-L-arginine (L-NMMA) to assess endothelium-dependent constriction (EDVC). Using magnetic resonance imaging the degree of the cerebral arteries diameter change in the tomograms before and after drug administration was evaluated. The study revealed the cerebral arteries dysfunction in rat-smokers. Acetylcholine stimulation in the rat-smokers leaded to the pathological vasoconstriction. Test with nitroglycerin caused insufficient vasodilatation (p <0,05). Test with L-NMMA showed the abnormal vasodilation in contrast with the control group which set the expected EDVC. In 2 months after smoking cessation the pathological vasoconstriction remained in the acetylcholine test. EIDVD was paradoxical even leading to the vasoconstriction. In long-term tobacco smoking the violation of the endothelial vasomotor function of the cerebral arteries has the form of the pathological activity of the vasodilating management and intensification of the vasoconstriction. After smoking cessation the vasomotor disturbances are not only preserved but also exarcebated.