@article {Abdul WahabP1118, author = {Atqah Abdul Wahab and Saad Taj-Aldeen and Ammar Saadoon and Sanjay Diophode and Corne Klaassen and Jacques Meis}, title = {Genotypic diversity of Pseudomonas aeruginosa in cystic fibrosis patients with the CFTR I1234V mutation in a large kindred family}, volume = {40}, number = {Suppl 56}, elocation-id = {P1118}, year = {2012}, publisher = {European Respiratory Society}, abstract = {Background: Pseudomonas aeruginosa is one of the primary pathogens within the cystic fibrosis (CF) lung and a cause of morbidity and mortality. Reports of cross infection and epidemics with this pathogen within and across CF centers raised the possibility of clonal spread among siblings with CF.Aim: To genotype P. aeruginosa by amplified fragment-length polymorphism (AFLP) analysis in CF patients with the CFTR I1234V mutation belonging to a large kindred tribe, and to determine whether the genotypes are identical among CF siblings and among different families with the same mutation.Methods: Sixty seven P aeruginosa isolates from sputa of 27 CF patients were studied at Hamad General Hospital, Qatar. Genotypic relatedness was assessed using AFLP, and compared with 33 P. aeruginosa isolates of a known genotype from the Netherlands.Results: Twenty seven CF patients were from 17 families, 16 males with a median age of 15 years (range 4 years-34 years). Twenty-two CF patients (12 families) with the CFTR I1234V mutation arising from a single large kindred tribe harboured chronic P. aeruginosa colonization. Twenty three unique genotype of P. aeruginosa were identified and different from P. aeruginosa strains isolated from Dutch CF patients. Ten families each had one unique genotype which were different from each other, four families harboured 2 different genotypes, one family 3 genotypes and two families had 4 genotypes. CF siblings within one family harboured the same genotypes of P.aeruginosa.Conclusions: This study demonstrated genotypic diversity of P. aeruginosa,isolates between families and established cross-infection among CF siblings.}, issn = {0903-1936}, URL = {https://erj.ersjournals.com/content/40/Suppl_56/P1118}, eprint = {https://erj.ersjournals.com/content/40/Suppl_56/P1118.full.pdf}, journal = {European Respiratory Journal} }