TY - JOUR T1 - Resveratrol attenuates cigarette smoke induced oxidative stress: Possible involvement of SIRT1 JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P4587 AU - Jing An AU - Yong-Chun Shen AU - Tao Wang AU - Ting Yang AU - Fu-Qiang Wen Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P4587.abstract N2 - Objectives: Cigarette smoke is known to cause oxidative stress in alveolar epithelial cells. In this study, we investigated the effects of resveratrol, a phytoalexin produced by some spermatophytes, on oxidative stress induced by cigarette smoke in airway epithelial cells.Methods: Rats with or without exposure to cigarette smoke were intraperitonealy injected with resveratrol (5,10,20mg/kg·d). TNF-α levels in BALF were determined by ELISA. Total glutathione, SOD and H2O2 levels in rat lungs were detected. Human alveolar epithelial cells (A549) were exposed to cigarette smoke extract (CSE), with or without pretreatment of resveratrol (40μM). The activity of ROS was detected and the expression of SIRT1 protein was evaluated with western blotting.Results: Cigarette smoke exposure significantly increased TNF-α expression in BALF and this upregulaion was significantly attenuated by resveratrol (p<0.05). Meanwhile, the treatment of resveratrol increased the expression of glutathione and SOD in lung homogenates, which were attenuated by cigarette smoke exposure (p<0.05). The expression of H2O2 was decreased by resveratrol(p<0.05). Exposure of A549 cells to CSE resulted in the elevated ROS expression, which was inhibited by resveratrol. Meanwhile, SIRT1 protein levels were activated by the pre-treatment of resveratrol.Conclusions: These results suggest that resveratrol attenuated oxidative stress induced by cigarette smoke. The anti-oxidant effect might act through the expression of SIRT1 proteins. ER -