RT Journal Article
SR Electronic
T1 Estimation of activity apoptosis genes based on expression of Bcl2, Bax, caspase-3 activity in bronchial epithelium in asthma
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P805
VO 40
IS Suppl 56
A1 Mineev, Valeriy
A1 Nesterovich, Irina
A1 Trofimov, Vasiliy
A1 Kashintseva, Tatiana
A1 Miroshkina, Alexandra
A1 Rybakova, Margarita
A1 Grozov, Roman
A1 Baikov, Vadim
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P805.abstract
AB Aim: The aim is to reveal the disorders of apoptosis in bronchial epithelium in bronchial asthma (BA) based on the estimation of expression of Bcl2, Bax, caspase-3.Methods: In 21 patients a fibreoptic bronchoscopy was performed (patients have signed the ICF). Expression of Bcl2, Bax, CPP32 (caspase-3 activity) in bronchial epithelium was performed by immunohistochemical analysis of bronchus biopsies taken in fibrobronchoscopy using DAKO kits.Results: In allergic BA elevation of Bcl2 expression and decrease of Bax expression compared to nonallergic BA and oral glucocorticoids taking patients were found. Activity of Bax expression was significant decreased in allergic BA compared to that in other groups. The same data were revealed on analysis of Bcl-2/Bax index. Expression level of caspase-3 was high in both groups.Conclusion: Features of apoptosis in bronchial epithelium in different variants of BA could indicate to different pathogenetic mechanisms of apoptosis in allergic inflammation persistence.