PT  - JOURNAL ARTICLE
AU  - Mineev, Valeriy
AU  - Nesterovich, Irina
AU  - Trofimov, Vasiliy
AU  - Kashintseva, Tatiana
AU  - Miroshkina, Alexandra
AU  - Rybakova, Margarita
AU  - Grozov, Roman
AU  - Baikov, Vadim
TI  - Estimation of activity apoptosis genes based on expression of Bcl2, Bax, caspase-3 activity in bronchial epithelium in asthma
DP  - 2012 Sep 01
TA  - European Respiratory Journal
PG  - P805
VI  - 40
IP  - Suppl 56
4099  - http://erj.ersjournals.com/content/40/Suppl_56/P805.short
4100  - http://erj.ersjournals.com/content/40/Suppl_56/P805.full
SO  - Eur Respir J2012 Sep 01; 40
AB  - Aim: The aim is to reveal the disorders of apoptosis in bronchial epithelium in bronchial asthma (BA) based on the estimation of expression of Bcl2, Bax, caspase-3.Methods: In 21 patients a fibreoptic bronchoscopy was performed (patients have signed the ICF). Expression of Bcl2, Bax, CPP32 (caspase-3 activity) in bronchial epithelium was performed by immunohistochemical analysis of bronchus biopsies taken in fibrobronchoscopy using DAKO kits.Results: In allergic BA elevation of Bcl2 expression and decrease of Bax expression compared to nonallergic BA and oral glucocorticoids taking patients were found. Activity of Bax expression was significant decreased in allergic BA compared to that in other groups. The same data were revealed on analysis of Bcl-2/Bax index. Expression level of caspase-3 was high in both groups.Conclusion: Features of apoptosis in bronchial epithelium in different variants of BA could indicate to different pathogenetic mechanisms of apoptosis in allergic inflammation persistence.