PT  - JOURNAL ARTICLE
AU  - Shuchang An
AU  - Jichao Chen
AU  - Zhanwei Wang
AU  - Xiaorong Wang
AU  - Xixin Yan
AU  - Jihong Li
AU  - Yusheng Chen
AU  - Jingping Yang
AU  - Xiaoling Xu
AU  - Jiabin Li
AU  - Qi Wang
AU  - Hui Wang
AU  - Zhancheng Gao
TI  - The mutation characteristics of the <em>bla</em> <sub>CTX-M/SHV/TEM</sub> -producing <em>Klebsiella pneumoniae</em> clinical isolates in multicenter in China
DP  - 2012 Sep 01
TA  - European Respiratory Journal
PG  - P2570
VI  - 40
IP  - Suppl 56
4099  - http://erj.ersjournals.com/content/40/Suppl_56/P2570.short
4100  - http://erj.ersjournals.com/content/40/Suppl_56/P2570.full
SO  - Eur Respir J2012 Sep 01; 40
AB  - Objectives Due to the prevalence of CTX-M- and SHV-type extended-spectrum β-lactamase (ESBL) -producing Enterobacteriaceae in China, the base mutation characteristics of blaCTX-M/SHV/TEM–producing Klebsiella pneumoniae were investigated.Methods From February 2010 to July 2011, 158 K. pneumoniae isolates from lower respiratory tract infection patients with positive double-disk synergy tests were collected from Beijing (69), Fujian (27), Anhui (22), Hebei (15), Liaoning (14), and Inner Mongolia (11). Ninety percent isolates were collected from common wards more than 48h after hospitalization. The blaCTX-M/SHV/TEM genes were examined by PCR and sequencing. The nucleotide sequences were analysed via BLAST program.Results In total, 138 blaCTX-M were detected, including 78 blaCTX-M-14, 19 blaCTX-M-15, 17 blaCTX-M-27, 12 blaCTX-M-3, 4 blaCTX-M-55, 2 blaCTX-M-65, 2 blaCTX-M-24, 2 blaCTX-M-24a, 1 blaCTX-M-38, and 1 blaCTX-M-98. 120 blaSHV were detected, including 45 blaSHV-11, 23 blaSHV-1, 15 blaSHV-12, 7 blaSHV-5, 5 blaSHV-108, 4 blaSHV-28, 3 blaSHV-36, 3 blaSHV2a, 2 blaSHV-2, 2 blaSHV-27, 2 blaSHV-142, 1 blaSHV-1a, 1 blaSHV-26, 1 blaSHV-32, 1 blaSHV-33, 1 blaSHV-38, 1 blaSHV-60, 1 blaSHV-103, 1 blaLEN, and 1 blaLEN-22. Also, 91 blaTEM-1 and 1 blaTEM-135 were detected. 8 blaSHV and 1 blaTEM-135 with the silent base mutations were detected, and none of the blaCTX-M.Conclusions The CTX-M-14, CTX-M-15, and CTX-M-27 were predominant ESBL genes in K. pneumoniae in China. The diversity of blaSHV and blaCTX-M, most silent mutations in blaSHV, and only missense mutations in blaCTX-M may be of significance contributing to epidemiological and clinical challenges.