TY - JOUR T1 - IL-4, IFN-gamma and TNF-alpha levels in serum of patients with COPD, bronchial asthma and GERD JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P796 AU - Kirill Sysoev Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P796.abstract N2 - Gastroesophageal reflux disease (GERD) is tightly linked to bronchial asthma and COPD. In our study we evaluated serum of 54 patients. Diagnoses was as follows: asthma (n=14), COPD (n=12), GERD (n=20), asthma and GERD (n=8). Serum of 19 volunteers was studied as a control group. IL-4, IFN-gamma and TNF-alpha levels were detected by ELISA. All patients underwent upper gastrointestinal endoscopy and spirometry. For statistics nonparametric method of Kruskal-Wallis and Spearman's correlation were used. Results. The IL-4 and IFN-gamma levels in all groups of patients were significantly higher than control (p=2.5E-09 and 1.76E-09 respectively). Meanwhile TNF-alpha values in patients cohorts was lower than in control group (p=0.007). Patients were divided into three groups according to endoscopy: (i) no symptoms (n=6), (ii) chronic gastritis (n=27), and (iii) reflux esophagitis (n=21). The level of IFN-gamma was lowest in no symptoms group (91.6±41.9 pg/mL), intermediate in gastritis patients (204.2±255.8 pg/mL), and highest in reflux eosophagitis group (404.8±455.7 pg/mL) — p=0.01. IFN-gamma/IL-4 ratio had similar dynamics (p=0.01). TNF-alpha value was maximal in reflux esophagitis group (p=0.0004). We investigated cytokines values depending on severity of respiratory failure. In patients with no respiratory failure level of IL-4 was minimal, stage 1 patients had medium elevation of IL-4 value, and in patients with stage 2 of RF level of cytokine was maximal (p=0.001). Conclusion. In our study IL-4 exhibited a significant role in the severity of respiratory disorders, whereas IFN-gamma and TNF-alpha were determined degree of damage to the gastrointestinal tract. ER -