RT Journal Article
SR Electronic
T1 Screening for biomarkers in pulmonary hypertension
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P3165
VO 40
IS Suppl 56
A1 Svenja Lena Tiede
A1 Soni Savai Pullamsetti
A1 Akylbek Sydykov
A1 Günter Lochnit
A1 Henning Tiede
A1 Hossein Ardeschir Ghofrani
A1 Norbert Weissmann
A1 Friedrich Grimminger
A1 Werner Seeger
A1 Ralph Theo Schermuly
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P3165.abstract
AB Introduction: Pulmonary hypertension (PH) is a progressive and fatal disease. The gold standard for diagnosing PH and estimating prognosis is the invasive method of right heart catheterization. To date no biomarker is available to prove or exclude the diagnosis of PH.Aims and objectives: The aim of this study is to identify and validate new biomarkers for PH.Methods: Plasma from the pulmonary artery banding (PAB) and the monocrotaline (MCT) rat model, and corresponding sham and control animals (n=9), was used for 2D-gel electrophoresis (2D-GE) and MALDI-TOF-MS analysis. Further, plasma changes of interesting candidates were confirmed by ELISA. Human study population consists of patients with idiopathic pulmonary arterial hypertension (n=40), PH associated with collagen vascular disease (n=45), pulmonary venous hypertension (n=44), chronic thromboembolic PH (n=45), and non-PH controls (n=34).Results: The spot density analysis of 2D-GE and identification by MALDI-TOF-MS revealed 7 proteins significantly changed in PAB vs. sham, and 15 proteins in MCT vs. control group. Complement component 4 (C4) and complement inhibitory factor H (CFH) were upregulated in PAB and MCT. ApoE was changed 15-fold in MCT plasma, but not in PAB. The analysis of the human samples revealed no significant difference in mean plasma ApoE between the patient groups (119.4±10.3, 147.6±11.6, 116.8±9.9, 110.2±8.3 µg/ml) and controls (135.3±14 µg/ml).Conclusions: Despite published data on the role of ApoE in PH and the significant changes in rats, ApoE seems not suitable as biomarker for PH in humans. Other candidates identified by mass spectrometry will be evaluated for their potential as biomarker.