RT Journal Article
SR Electronic
T1 Effect of cigarette smoke on peripheral blood Th17 (PBTh17) cells from COPD patients. Role of acetylcholine
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P1427
VO 40
IS Suppl 56
A1 Profita, Mirella
A1 Allbano, Giusy Daniela
A1 Di Sano, Caterina
A1 Montalbano, Angela Marina
A1 Gagliardo, Rosalia
A1 Anzalone, Giulia
A1 Riccobono, Loredana
A1 Bonanno, Anna
A1 Pieper, Michael Paul
A1 Gjomarkaj, Mark
YR 2012
UL https://publications.ersnet.org//content/40/Suppl_56/P1427.abstract
AB PBTh17 cells producing IL-17A and IL-22, as well as Acetylcholine (ACh) are involved in the systemic inflammation of several autoimmune and inflammatory diseases, including COPD.We investigated the effect of cigarette smoke extract (CSE) and the role of ACh on the expression of RORgt, IL-17A and IL-22 in PBTh17 cells during systemic inflammation in COPD.ACh, IL-17A, IL-22 and RORgt levels as well as the colocalization of ACh with IL-17A, IL-22 and RORgt were evaluated (flowcytometry) in PBT-lymphocytes (PBT) from COPD patients (n=16), healthy smokers (HS) (n=12) and healthy control subjects (HC) (n=13). Furthermore, PBT cells from COPD (n=6) patients and PBT cells from HC (n=6) stimulated in vitro with CSE (10 %) were cultured for 72 hrs in the presence or absence of Tiotropium (Spiriva®) (20 nM) and Olodaterol (1 nM) alone or in combination.The colocalization of ACh with IL-17A, IL-22 and RORgt was significantly increased in PBT cells from COPD patients when compared to HC and HS subjects as well as in PBT cells from HC stimulated with CSE when compared with unstimulated cells. Tiotropium and Olodaterol alone reduced the increased levels of colocalization between ACh with IL-17A, IL-22 and RORgt in cultured PBT-cells from COPD patients and in PBT-cells from HC subjects stimulated with CSE.We suggests that cigarette smoke might be able to increase the levels of ACh in PBT cells promoting the switch into PBTh17 cells which produce IL-17A and IL-22 during the systemic inflammation in COPD. The use of anticholinergics as Tiotropium and long-acting β2-agonists as Olodaterol might prevent these events.Funded by: Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach, Germany.