PT  - JOURNAL ARTICLE
AU  - Kopinski, Piotr
AU  - Wandtke, Tomasz
AU  - Chorostowska-Wynimko, Joanna
AU  - Przybylski, Grzegorz
AU  - Pólgesek, Ewelina
AU  - Dyczek, Andrzej
TI  - Expression of interleukin-27 (IL27) in human lower airways. Pathophysiological implications in pulmonary sarcoidosis (PS)
DP  - 2012 Sep 01
TA  - European Respiratory Journal
PG  - P3613
VI  - 40
IP  - Suppl 56
4099  - http://erj.ersjournals.com/content/40/Suppl_56/P3613.short
4100  - http://erj.ersjournals.com/content/40/Suppl_56/P3613.full
SO  - Eur Respir J2012 Sep 01; 40
AB  - Background: Interleukin-27 was recently discovered as a cytokine secreted by antigen presenting cells, participating in T cell priming and supporting Th1 type immune polarization. IL27 expression has not been examined yet in lower airways.Aims: Identification of IL27 sources in human lower airways. Preliminary assessment of IL27 expression in bronchoalveolar lavage (BAL) in interstitial lung diseases (ILD) patients.Methods: Intracellular IL27 expression cells was determined by indirect phenotyping and flow cytometry in BAL cells obtained from patients with PS (incl. steroid treated subjects), idiopathic pulmonary fibrosis (IPF) and controls, as well as in Human Lung Fibroblasts (HLF1) and pneumocyte type II (A549) cell lines. IL27 extracellular secretion was tested by ELISA (cat. no E90385Hu, Diaclone) in supernatants of BAL and cell cultures.Results: BAL detectable IL27 levels were shown by ELISA in 6 (median 9ng/ml) of 13 PS untreated patients. IL27 was not found in BAL supernatants of steroid-treated PS, IPF and controls. HLF1, A549 and alveolar macrophages were positive for intracellular IL27. Surprisingly, IL27+ BAL lymphocytes were found in all tested groups (PS: 81±6.2%; IPF: 37±13.4%; controls: 46±15%, median±SEM, insignificant).Conclusions: In physiological conditions, IL27 is produced in lower airways by lung fibroblasts, epithelial and BAL immune cells, including lymphocytes. Its increased expression in PS suggests IL27 to play a role in ILD pathophysiology, probably as Th1 activity marker.