RT Journal Article
SR Electronic
T1 The in vitro effect of vitamin D on peripheral blood mononuclear cell cytokine expression in COPD
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P2396
VO 40
IS Suppl 56
A1 Stewart, Niall
A1 Wood-Baker, Richard
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P2396.abstract
AB BackgroundVitamin D is recognised as a powerful modulator of immune responses. It promotes T helper 2 (Th2) immunity and the anti-inflammatory cytokine Interleukin-10 (IL-10). However, little is known about the effects of vitamin D on the immune response in COPD, a Th1 mediated disease.Aims and objectivesWe hypothesised that in COPD vitamin D would push a Th2 response, with increased levels of the Th2 cytokine Interleukin-4 (IL-4) and the suppressive cytokine IL-10. Such a shift could be beneficial in COPD.MethodsWe recruited 10 COPD subjects from whom we isolated peripheral blood mononuclear cells (PBMC) and measured serum 25(OH)D. PBMC were stimulated with antibodies to the T cell receptor either with or without added vitamin D.ResultsFor both IL-4 and IL-10, we observed a biphasic effect of vitamin D supplementation. For those with low (<30nmol/L) and high (>80nmol/L) serum 25(OH)D, added vitamin D suppressed IL-4 and IL-10 (Figure 1,2). Between these two levels, vitamin D increased expression.ConclusionsSerum 25(OH)D levels may be an important determinant of the usefulness of vitamin D in beneficially modifying the immune response in COPD.