RT Journal Article
SR Electronic
T1 Respiratory syncytial virus (RSV) causes ciliary dyskinesia but not loss of frequency during infection of human ciliated respiratory epithelial cells in culture
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P1918
VO 40
IS Suppl 56
A1 Claire Smith
A1 Gwyneth Williams
A1 Andrew Rutman
A1 Andrew Easton
A1 Peter Andrew
A1 Chris O'Callaghan
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P1918.abstract
AB Aim To determine the effect of RSV on ciliary function using ciliated air–liquid interface (ALI) cultures.Introduction: RSV targets ciliated respiratory epithelial cells for infection1. Two previous studies on the effect of RSV on ciliated cultures have shown different effects, one describing rapid ciliostasis2 and another describes no cytopathic effect1.Methods Ciliated epithelial cells were cultured from nasal and bronchial brush biopsies. Cells were infected with RSV (A2) for up to 72h. Ciliary beat frequency (CBF) and pattern (CBP) were observed with a high-speed video camera. The cells were fixed and stained using anti-RSV (FITC) and anti-acetylated tubulin (Alexa594) antibodies. Confocal optical sectioning and electron microscopy (EM) was also performed.Results CBP showed a higher proportion of dyskinetic cilia following RSV infection. The median (IQR) dyskinesia index was increased as early as 24h and at 72h had increased to 33% (25-42%) compared with controls 13% (11-14%). CBF was unaffected by RSV infection. By 72h the ultrastructure of the ciliated epithelium was abnormal following RSV infection with an increased in ciliated cells exhibiting loss of cilia and an increase in ciliated cells with mitochondrial damage. Furthermore, RSV antigens were observed on the apical surface of the cell and had extended the full length of the cilial shaft.Conclusion RSV infection of ciliated cells results in increased dyskinesia of cilia, whilst CBF is maintained. Epithelial damage was preferentially seen in ciliated cells.Refs1. J Virol. 2005;79(2):1113-11242. Arch Otolaryngol Head Neck Surg, 1998;124(7):777-83.