PT  - JOURNAL ARTICLE
AU  - Bruno Martins Oliveira
AU  - Rafael Almeida-Reis
AU  - Osmar A. Theodoro
AU  - Leandro V. Oliva
AU  - Daniel Flisch Rodrigues
AU  - Nathalia Pinheiro
AU  - Maria L.V. Oliva
AU  - Carla M. Prado
AU  - Milton M. Martins
AU  - Iolanda F.L.C. Tibério
TI  - Plant proteinase from Bauhinia bauhinioides Kallikrein inhibitor (BbKI) attenuates mechanics, inflammation and remodelling induced by elastase in mice
DP  - 2012 Sep 01
TA  - European Respiratory Journal
PG  - P826
VI  - 40
IP  - Suppl 56
4099  - http://erj.ersjournals.com/content/40/Suppl_56/P826.short
4100  - http://erj.ersjournals.com/content/40/Suppl_56/P826.full
SO  - Eur Respir J2012 Sep 01; 40
AB  - Proteinases plays a key role on emphysema development. This study evaluated the capacity of the plant proteinase inhibitor BbKI in the inactivation of elastase and its response modulator. Methods: C57Bl6 mice received elastase intratracheal or saline (Ve group). Afterwards, mice were treated with BbKI (2mg/kg) on days 1, 14, 21 after elastase instillation (I-E group) or saline instilation. On day 30 mice were anesthetized and mechanically ventilated and we analyzed respiratory system resistance (Rrs), elastance (Ers), tissue elastance (Htis), tissue damping (Gtis), airway resistance (Raw) and exhaled nitric oxide (ENO). Afterwards, bronchoalveolar lavage fluid (BALF) was performed and lungs were removed. By morphometry, we quantified the mean linear intercept (Lm), and the amount of collagen and elastic fibers in distal lung parenchyma. Results: In elastase group there was a significant increase in the Ers, Rrs, Raw, Htis, Lm, ENO, total and, macrophages, neutrophils and lymphocytes in BALF, and elastic and collagen fibres compared to controls (p<0.05). The BbKI treatment of elastase group decreased the Lm (59.33±4.74µm), Raw (0.33±0.05cmH2O/ml/s), Ers (36.83±5.73cmH2O/L), Rrs (0.843±0.19cmH2O/mL/s), Htis (37.360±6.2cmH2O/mL/s), total cells (69.25±20.98x104cells/mL), neutrophils (19.38±9.11x104cells/mL), lymphocytes (1.95±1.24x104cells/mL) in the BALF, ENO (19,66±8,33ppb) and elastic fibers content (30%±0.1%) compared to E-group (p<0.05). Conclusions: This proteinase inhibitor (BBKI) reduced elastase-induced pulmonary inflammatory and extracellular matrix remodeling alterations. Financial Support: FAPESP, CNPq, LIM-20 HCFMUSP.