%0 Journal Article %A Eivind Brønstad %A Jose Bianco %A Ulrik Wisløff %A Sigurd Steinshamn %T Effects of exercise on ubiquitination and proteasome activity in skeletal muscle in COPD patients %D 2012 %J European Respiratory Journal %P 4701 %V 40 %N Suppl 56 %X OBJECTIVE: COPD patients have reduced exercise capacity and skeletal muscle function. The aim of this study was to investigate exercise effects on proteasome actvity in skeletal muscle of COPD patients. We wanted to evaluate ventilatory limited and unlimited exercise by comparing whole body training against small isolated muscle training. The effects of oxidative stress and systemic inflammation were also evaluated.METHODS: In total, 21 patients with moderate to severe COPD were studied. Spirometry, peak oxygen uptake (VO2-peak), ubiquitin activity, proteasome activity, protein carbonylation and systemic inflammation were measured before and after a 10 week aerobic exercise program (n=14) and a 6 week knee extensor exercise program (n=7). Five age- and sex-matched healthy individuals served as reference group.RESULTS: At baseline all COPD groups had reduced proteasome activity compared to controls (p<0.05). When comparing the effects of the two modes of exercise, only whole body aerobic exercise resulted in increased proteasome activity in both the aerobic interval and the moderate continuous group. We found no differences in carbonylation or protein ubiqutination. Serum levels of TNFa and IL-6 were not influenced by training.CONCLUSION: COPD patients have altered skeletal muscle proteasome activity and whole body aerobic exercise training induces an increased proteasome activity compared to isolated muscle training. The increase exclusively in the whole body exercise groups could therefore suggest an inappropriate skeletal muscle response, and could imply that training small muscle groups is better for COPD patients. %U https://erj.ersjournals.com/content/erj/40/Suppl_56/4701.full.pdf