RT Journal Article
SR Electronic
T1 Neutrophil extracellular traps in bronchial aspirates: a quantitative analysis
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 1709
OP 1718
DO 10.1183/09031936.00139813
VO 43
IS 6
A1 Shigeto Hamaguchi
A1 Tomoya Hirose
A1 Naoya Matsumoto
A1 Yukihiro Akeda
A1 Taro Irisawa
A1 Masafumi Seki
A1 Hideo Hosotsubo
A1 Kouji Yamamoto
A1 Osamu Tasaki
A1 Kazunori Oishi
A1 Takeshi Shimazu
A1 Kazunori Tomono
YR 2014
UL http://erj.ersjournals.com/content/43/6/1709.abstract
AB Neutrophil extracellular traps (NETs) are structures composed of DNA and granular proteins, which rapidly trap and kill pathogens. The formation of NETs has been detected during infection in animal experiments, but their role in humans is unclear. The purposes of this study were to quantitatively evaluate the production of NETs during acute respiratory infection and to study the relationship between the NET length and various inflammatory mediators. We examined bronchial aspirates collected from nine intubated patients in an intensive care unit. Samples were collected at the onset of acute respiratory infection (day 0) and on days 1, 3–5, and 6–8. The NET length was visualised by immunohistochemistry and quantified using computer tracing software. The NET length was measured and compared at each time point. The length differed significantly between time points (p<0.001). NETs were significantly longer on day 1 than on day 0 (p<0.001). Neutrophils released NETs abundantly in response to respiratory infection and regression analysis showed that NET length correlated with six clinical parameters (white blood cells, platelets, lactate, CXC ligand-2, interleukin-8, and procalcitonin) as the explanatory variables. NETs in bronchial aspirates may reflect disease progression of respiratory infections. Quantification of NETs in bronchial aspirates may provide a new indicator of inflammation. NET length increases with respiratory inflammation which correlates with progression of infections http://ow.ly/sPbEX